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bulk mrnaseq highly purified cd11b monocytes  (Miltenyi Biotec)


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    Miltenyi Biotec bulk mrnaseq highly purified cd11b monocytes
    Bulk Mrnaseq Highly Purified Cd11b Monocytes, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+monocytes/CD11b%2Fc+Antibody%2C+anti-rat%2C+REAfinity/pm42007695-262-6-27
    Average 98 stars, based on 26 article reviews
    bulk mrnaseq highly purified cd11b monocytes - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Purification:

    Article Title: HIV transgenic mouse monocytes display increased in vivo migration across the blood-brain barrier associated with increased expression of genes associated with mononuclear leukocyte movement.
    Article Snippet: .. In some experiments, highly purified CD11b+ monocytes (>90% CD11b+) were isolated from the bone marrow leukocytes after 1× RBC lysis by immunomagnetic separation using CD11b mouse MicroBeads and a MiniMACS Separator column (Miltenyi Biotec, Gaithersburg, MD) and then washed and resuspended in PBS as described (81). .. Evaluation of in vivo migration of bone marrow leukocytes across the blood-brain barrier Prior to the intravenous injection of BML from donor JRCCC or CCC mice, recipient C57BL/6 mice (B6 mice) were either intraperitoneally injected with LPS (3 mg/kg) from Escherichia coli O55:B5 (Sigma-Aldrich, Saint Louis, MO) to increase the permeability of the BBB or with PBS as a negative control.

    Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis
    Article Snippet: Data acquisition and analysis were done using LSR II flow cytometry and FlowJo software, respectively. .. CD11b + monocytes were isolated and purified from mouse bone marrow cells using CD11b MicroBeads UltraPure mouse magnetic bead (Miltenyi Biotec) according to the manufacturer’s protocol. .. Isolated monocytes (1 × 10 6 cells/mL) were differentiated into dendritic cells by 6 days of culturing with RPMI 1640 medium supplemented with 10% FBS, 1% penicillin/streptomycin, 10 mM HEPES, 50 μM 2-mercaptoethanol (Thermo Fisher Scientific), 20 ng/mL of IL-4 (Thermo Fisher Scientific) and 100 ng/mL of GM-CSF (Thermo Fisher Scientific) in 6-well tissue culture plates (Corning Costar).

    Isolation:

    Article Title: HIV transgenic mouse monocytes display increased in vivo migration across the blood-brain barrier associated with increased expression of genes associated with mononuclear leukocyte movement.
    Article Snippet: .. In some experiments, highly purified CD11b+ monocytes (>90% CD11b+) were isolated from the bone marrow leukocytes after 1× RBC lysis by immunomagnetic separation using CD11b mouse MicroBeads and a MiniMACS Separator column (Miltenyi Biotec, Gaithersburg, MD) and then washed and resuspended in PBS as described (81). .. Evaluation of in vivo migration of bone marrow leukocytes across the blood-brain barrier Prior to the intravenous injection of BML from donor JRCCC or CCC mice, recipient C57BL/6 mice (B6 mice) were either intraperitoneally injected with LPS (3 mg/kg) from Escherichia coli O55:B5 (Sigma-Aldrich, Saint Louis, MO) to increase the permeability of the BBB or with PBS as a negative control.

    Article Title: Interleukin-33 Ameliorates Murine Systemic Lupus Erythematosus and Is Associated with Induction of M2 Macrophage Polarisation and Regulatory T Cells
    Article Snippet: Immune cells were isolated from the kidneys digested with collagenase IV (Sigma, USA) followed by Ficoll-Paque density gradient centrifugation. .. Splenocytes were isolated as described earlier from which CD11b+ monocytes were extracted by magnetic isolation (Miltenyi Biotec, USA). .. Cells were incubated with mouse Fc block (Miltenyi) at 4°C followed by fluorochrome-labelled monoclonal antibodies and corresponding isotypic controls: anti-mouse CD3-APC, CD4-PE, CD4-APC, CD4-FITC, CD8-APCCy7, CD11c-FITC, CD11b-PE, CD25-PE, ST2-APC, CD206-PECy7 (BioLegend), CD19-PECy7, inducible nitric oxide synthase (iNOS)-APC (eBioscience, USA).

    Article Title: 2′–5′ oligoadenylate synthetase‑like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability
    Article Snippet: AccuTarget miRNA Negative Control, mimic (SMC-2001) and inhibitor (SMC-2101), as well as human miRNA-584 mimic (SMM-003) and miRNA-584-5p inhibitor (SMI-002), were purchased from Bioneer (Korea) and used at a concentration of 80 nM. .. CD11b + monocytes were isolated from the BM of Oasl1 +/+ and Oasl1 −/− mice using CD11b Ab-conjugated MACS beads (Miltenyi Biotec, 130-049-601). .. Following RBC lysis, the BM cells were resuspended in 90 μl of MACS buffer (PBS pH 7.2, containing 0.5% BSA and 2 mM EDTA), then 10 μl of CD11b MicroBeads were added to each sample (per 1 × 10 7 total cells).

    Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis
    Article Snippet: Data acquisition and analysis were done using LSR II flow cytometry and FlowJo software, respectively. .. CD11b + monocytes were isolated and purified from mouse bone marrow cells using CD11b MicroBeads UltraPure mouse magnetic bead (Miltenyi Biotec) according to the manufacturer’s protocol. .. Isolated monocytes (1 × 10 6 cells/mL) were differentiated into dendritic cells by 6 days of culturing with RPMI 1640 medium supplemented with 10% FBS, 1% penicillin/streptomycin, 10 mM HEPES, 50 μM 2-mercaptoethanol (Thermo Fisher Scientific), 20 ng/mL of IL-4 (Thermo Fisher Scientific) and 100 ng/mL of GM-CSF (Thermo Fisher Scientific) in 6-well tissue culture plates (Corning Costar).

    Article Title: 2'-5' oligoadenylate synthetase‑like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability.
    Article Snippet: AccuTarget miRNA Negative Control, mimic (SMC-2001) and inhibitor (SMC-2101), as well as human miRNA-584 mimic (SMM-003) and miRNA-584-5p inhibitor (SMI-002), were purchased from Bioneer (Korea) and used at a concentration of 80 nM. .. CD11b+ monocytes were isolated from the BM of Oasl1+/+ and Oasl1−/− mice using CD11b Ab-conjugated MACS beads (Miltenyi Biotec, 130- 049-601). ..

    Red Blood Cell Lysis:

    Article Title: HIV transgenic mouse monocytes display increased in vivo migration across the blood-brain barrier associated with increased expression of genes associated with mononuclear leukocyte movement.
    Article Snippet: .. In some experiments, highly purified CD11b+ monocytes (>90% CD11b+) were isolated from the bone marrow leukocytes after 1× RBC lysis by immunomagnetic separation using CD11b mouse MicroBeads and a MiniMACS Separator column (Miltenyi Biotec, Gaithersburg, MD) and then washed and resuspended in PBS as described (81). .. Evaluation of in vivo migration of bone marrow leukocytes across the blood-brain barrier Prior to the intravenous injection of BML from donor JRCCC or CCC mice, recipient C57BL/6 mice (B6 mice) were either intraperitoneally injected with LPS (3 mg/kg) from Escherichia coli O55:B5 (Sigma-Aldrich, Saint Louis, MO) to increase the permeability of the BBB or with PBS as a negative control.

    Immunomagnetic Separation:

    Article Title: HIV transgenic mouse monocytes display increased in vivo migration across the blood-brain barrier associated with increased expression of genes associated with mononuclear leukocyte movement.
    Article Snippet: .. In some experiments, highly purified CD11b+ monocytes (>90% CD11b+) were isolated from the bone marrow leukocytes after 1× RBC lysis by immunomagnetic separation using CD11b mouse MicroBeads and a MiniMACS Separator column (Miltenyi Biotec, Gaithersburg, MD) and then washed and resuspended in PBS as described (81). .. Evaluation of in vivo migration of bone marrow leukocytes across the blood-brain barrier Prior to the intravenous injection of BML from donor JRCCC or CCC mice, recipient C57BL/6 mice (B6 mice) were either intraperitoneally injected with LPS (3 mg/kg) from Escherichia coli O55:B5 (Sigma-Aldrich, Saint Louis, MO) to increase the permeability of the BBB or with PBS as a negative control.

    Magnetic Beads:

    Article Title: Vista modulators for diagnosis and treatment of cancer
    Article Snippet: .. For sorting CD11bhi myeloid APCs, CD11b+ monocytes were enriched from naive splenocytes using CD11b magnetic beads (Miltenyi Biotec). ..

    Article Title: Vista modulators for diagnosis and treatment of cancer
    Article Snippet: .. For sorting CD11bhi myeloid APCs, CD11b+ monocytes were enriched from naive splenocytes using CD11b magnetic beads (Miltenyi Biotec). ..

    Article Title: VISTA-Ig constructs and the use of VISTA-Ig for treatment of autoimmune, allergic and inflammatory disorders
    Article Snippet: .. For sorting CD11bhi myeloid APCs, CD11b+ monocytes were enriched from naive splenocytes using CD11b magnetic beads (Miltenyi Biotec). ..

    Magnetic Cell Separation:

    Article Title: 2′–5′ oligoadenylate synthetase‑like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability
    Article Snippet: AccuTarget miRNA Negative Control, mimic (SMC-2001) and inhibitor (SMC-2101), as well as human miRNA-584 mimic (SMM-003) and miRNA-584-5p inhibitor (SMI-002), were purchased from Bioneer (Korea) and used at a concentration of 80 nM. .. CD11b + monocytes were isolated from the BM of Oasl1 +/+ and Oasl1 −/− mice using CD11b Ab-conjugated MACS beads (Miltenyi Biotec, 130-049-601). .. Following RBC lysis, the BM cells were resuspended in 90 μl of MACS buffer (PBS pH 7.2, containing 0.5% BSA and 2 mM EDTA), then 10 μl of CD11b MicroBeads were added to each sample (per 1 × 10 7 total cells).

    Article Title: 2'-5' oligoadenylate synthetase‑like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability.
    Article Snippet: AccuTarget miRNA Negative Control, mimic (SMC-2001) and inhibitor (SMC-2101), as well as human miRNA-584 mimic (SMM-003) and miRNA-584-5p inhibitor (SMI-002), were purchased from Bioneer (Korea) and used at a concentration of 80 nM. .. CD11b+ monocytes were isolated from the BM of Oasl1+/+ and Oasl1−/− mice using CD11b Ab-conjugated MACS beads (Miltenyi Biotec, 130- 049-601). ..



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    Image Search Results


    ( A ) Left: Ingenuity Pathways Analysis of predicted upstream regulators for macrophages cultured with WT relative to P2rx4 -KO fibroblasts (fib). Right: CellChat plot comparing WT and P2rx4 -KO conditions. Significance was determined by Wilcoxon’s test, with P < 0.05 being statistically significant. Data represent 2 separate cocultures. ( B ) Violin plot for Il6 expression in WT relative to P2rx4 -KO fibroblasts from cocultures. Line shows median values. Data represent 2 separate cocultures. ( C ) ARG1 ELISA of cell lysates and conditioned media collected from mouse lung macrophage (mac) monoculture 72 hours after IL-6 treatment. n = 3 biological replicates per condition. *** P < 0.001, **** P < 0.0001 by Student’s t test. ( D ) Left: ARG1 ELISA of BAL fluid normalized to lung CD11B + CD64 + macrophage count taken at day 14 from bleomycin-injured WT or Il6 -KO mice. Right: Arg1 qPCR of CD11B + CD64 + macrophages. n = 5 mice per condition. ** P < 0.01 by Student’s t test. ( E ) Quantification of Il6 expression by cell type in cells that underwent scRNA-Seq directly after isolation from the lung at steady state and multiple time points after injury: reanalysis of merged data from Strunz et al. and Tsukui et al. normalized by Sctransform . ( F ) UMAP of fibroblast subtypes (left) and Il6 feature plots at steady state (center) and 7 days after bleomycin injury (right), from Tsukui et al. . ( C , D , and F ) Data are reported as mean ± SEM.

    Journal: The Journal of Clinical Investigation

    Article Title: Myeloid-mesenchymal crosstalk drives ARG1-dependent profibrotic metabolism via ornithine in lung fibrosis

    doi: 10.1172/JCI188734

    Figure Lengend Snippet: ( A ) Left: Ingenuity Pathways Analysis of predicted upstream regulators for macrophages cultured with WT relative to P2rx4 -KO fibroblasts (fib). Right: CellChat plot comparing WT and P2rx4 -KO conditions. Significance was determined by Wilcoxon’s test, with P < 0.05 being statistically significant. Data represent 2 separate cocultures. ( B ) Violin plot for Il6 expression in WT relative to P2rx4 -KO fibroblasts from cocultures. Line shows median values. Data represent 2 separate cocultures. ( C ) ARG1 ELISA of cell lysates and conditioned media collected from mouse lung macrophage (mac) monoculture 72 hours after IL-6 treatment. n = 3 biological replicates per condition. *** P < 0.001, **** P < 0.0001 by Student’s t test. ( D ) Left: ARG1 ELISA of BAL fluid normalized to lung CD11B + CD64 + macrophage count taken at day 14 from bleomycin-injured WT or Il6 -KO mice. Right: Arg1 qPCR of CD11B + CD64 + macrophages. n = 5 mice per condition. ** P < 0.01 by Student’s t test. ( E ) Quantification of Il6 expression by cell type in cells that underwent scRNA-Seq directly after isolation from the lung at steady state and multiple time points after injury: reanalysis of merged data from Strunz et al. and Tsukui et al. normalized by Sctransform . ( F ) UMAP of fibroblast subtypes (left) and Il6 feature plots at steady state (center) and 7 days after bleomycin injury (right), from Tsukui et al. . ( C , D , and F ) Data are reported as mean ± SEM.

    Article Snippet: In the case of quantifying and sorting CD45 + CD11B + CD64 + monocyte-derived macrophages in bleomycin-injured WT and Il6 -KO mouse lung cell suspensions, 50,000 microbeads (Invitrogen/Thermo Fisher Scientific) were added to each sample for quantification of absolute live CD45 + CD11b + CD64 + cells.

    Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, Isolation